Immobilized enzyme reactors in HPLC and its application in inhibitor screening: A review

نویسندگان

  • Si-Meng Fang
  • Hai-Na Wang
  • Zhong-Xi Zhao
  • Wei-Hong Wang
چکیده

This paper sets out to summarize the literatures based on immobilized enzyme bio-chromatography and its application in inhibitors screening in the last decade. In order to screen enzyme inhibitors from a mass of compounds in preliminary screening, multi-pore materials with good biocompatibility are used for the supports of immobilizing enzymes, and then the immobilized enzyme reactor applied as the immobilized enzyme stationary phase in HPLC. Therefore, a technology platform of high throughput screening is gradually established to screen the enzyme inhibitors as new anti-tumor drugs. Here, we briefly summarize the selective methods of supports, immobilization techniques, co-immobilized enzymes system and the screening model.

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عنوان ژورنال:

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2012